Informatics Publishing Limited

Mohit Gera , A. S. Bhandari , Tribhuvan Singh

Abstract

Root trainers are increasingly being used to deal with the problems of ischolar_main coiling and distortion, common to the polythene bag planting system. Experimental seedlings of three multipurpose tree species namely Acacia nilotica, Albizia procera and Dalbergia sissoo were raised in ischolar_main trainers (150 cc Hiko pots) in a randomized block design. The seedlings were assessed for quality parameters, viz. Sturdiness quotient, ischolar_main-shoot ratio, ratio of secondary + tertiary ischolar_main biomass to the total ischolar_main biomass and number of nodules. The quality of experimental seedlings was compared with seedlings raised in polythene bags kept on mounted angle iron beds. Root trainer raised seedlings were found to be better in sturdiness whereas seedlings raised in polythene bags showed higher nodulation. The results of the other two quality parameters assessed were comparable. No conclusive results could be drawn on the superiority of the two systems, whieh however, is possible once the seedlings are assessed for growth and survival after two to three years of fields planting.

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Tribhuvan Singh ¹, Harvinder Singh Saini ², S. V. Ranganayakulu ², S. Ravi ³, K. Sudhakar ³

Affiliations
1 Guru Nanak Institutions Technical Campus - School of Pharmacy Ibrahimpatnam, Telangana - 501506, IN
2 Guru Nanak Institutions Technical Campus - School of Engineering and Technology Ibrahimpatnam, Telangana - 501506, IN
3 Rural Development Society, R&D Center, Punjagutta, Hyderabad - 500082, Andhra Pradesh, IN

Abstract

Objectives: To isolate Azadirachtin-A, from Seeds of Azadirachta indica and to perform molecular docking studies for antiproliferative activity against cervical cancer. Methods: The Current studies on Azadirachtin-A, a potent cervical anticancer agent, was designed for the isolation of Azadirachtin-A, from neem fruits collected from local area (Ibrahimpatnam), the seeds were first separated, dried and made in to coarse powder. The seed kernels was defatted and extracted with methanol using soxhlet apparatus. Methanolic extract of seeds were subjected to direct reverse phase High Performance Liquid Chromatography (HPLC) for separation of Azadirachtin-A, by molecular docking of Azadirachtin-A, using Molecular Operating Environment (MOE) 2008 software Findings: This revealed that Azadirachtin-A, has an affinity of 59.2% and 72% towards amino acids like Glycine 131 and Lysine 89 with polar intractions. Azadirachtin-A, found to interact with Cyclin E, was retrieved from the Protein Data Bank (PDB code: 1W98). The Azadirachtin-A, showed excellent, Increase Life Span (63.44 and 82.56), reduced the viable cells (30.4 ± 1.5 and 24.5 ± 1.6), and tumor volume (1.2 ± 0.05 and 1.0 ± 0.05) when compared to standard drug 5-Fluorouracil. Application: Azadirachtin-A, showed significant docking interaction with Cyclin E, active site using windows 2002, MOE 2008 software. Result showed that Azadirachtin-A, may lead to potent cervical anticancer agent.

Keywords

Azadirachtin-A, Azadirachta indica, Cervical Cancer, 5-Fluorouracil, (HeLa) Henrietta Lacks Cell Line, Molecular Docking.

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